Chromatography Column Accessories

These materials separate proteins on the basis of hydrophobic character, as does Reversed-Phase Chromatography. HIC uses totally aqueous buffers, maintaining tertiary structure and biological activity. Typically, a sample is eluted with a decreasing gradient of a salt such as sulfate or phosphate. Proteins elute in order of increasing surface hydrophobicity. Surfactants (e.g. CHAPS, octylglucoside) can be added to the mobile phase if necessary. The relative hydrophobic character of PolyMETHYL A is 15.

When To Use HIC:

  1. Characterization of antibodies.
  2. Purification of polypeptides such as glycopeptides and venoms.
  3. Isolation of proteins from crude extracts.
  4. Quality control assay using a method complementary to ion-exchange and Reversed-Phase.
  5. Isolation of integral membrane proteins and their complexes.

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Item #

Item Name

Type

List Price

001001

CONNECTOR 2-WAY 3 PK

Connector

$63.80
001101

VALVE 2-WAY

Valve

$99.25
002507

VALVE 1/4-28 TO VARIABLE BORE

Adapter

$62.63
001010

CONNECTOR 3-WAY TEE

Connector

$69.71
001109

VALVE 3-WAY SINGLE KEY

Valve

$116.97
001131

VALVE COLUMN BLEED

Valve

$108.70
001003

CONNECTOR 3-WAY HEX

Connector

$69.71
001102

VALVE 3-WAY HEX

Valve

$174.87
001008

CONNECTOR UNIVERSAL

Connector

$89.81
001105

VALVE UNIVERSAL CONNECTOR

Connector

$74.44
002501

ADAPTOR MALE LUER

Adapter

$18.44
002502

ADAPTOR FEMALE LUER

Adapter

$18.44
001201

ADAPTOR MALE LUER ETFE

Adapter

$22.81
001202

ADAPTOR FEMALE LUER ETFE

Adapter

$22.81
001206

ADAPTOR 1/4-28 TO LUER LOCK

Adapter

$42.53
002310

GRIPPER 1/16

Gripper Fitting

$60.26
002312

GRIPPER 1/8

Gripper Fitting

$60.26
001502

CONES 1/16 (1.6MM) SMALL

Cone

$17.48
001503

PTFE Cones for 3/32" OD Tubing

Cone

$9.65
001506

CONES 0.5MM SMALL

Cone

$17.48
001507

CONES 1.0MM SMALL

Cone

$17.48
001508

CONES 1.5MM SMALL

Cone

$17.48
001509

CONES 2.0MM SMALL

Cone

$17.48
001510

CONES 2.5MM SMALL

Cone

$17.48
001512

CONES 3.5MM SMALL

Cone

$17.48