PolyMETHYL A Columns

These materials separate proteins on the basis of hydrophobic character, as does Reversed-Phase Chromatography. HIC uses totally aqueous buffers, maintaining tertiary structure and biological activity. Typically, a sample is eluted with a decreasing gradient of a salt such as sulfate or phosphate. Proteins elute in order of increasing surface hydrophobicity. Surfactants (e.g. CHAPS, octylglucoside) can be added to the mobile phase if necessary. The relative hydrophobic character of PolyMETHYL A is 15.

When To Use HIC:

  1. Characterization of antibodies.
  2. Purification of polypeptides such as glycopeptides and venoms.
  3. Isolation of proteins from crude extracts.
  4. Quality control assay using a method complementary to ion-exchange and Reversed-Phase.
  5. Isolation of integral membrane proteins and their complexes.

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Item #

Item Name

Particle Size

Pore Size

Column Inside Diameter

Column Length

List Price

051ME0503

PolyMethyl A 5um 300A 1 x 50mm

5 µm

300 A

1 mm

50 mm

$437.75
151ME0503

PolyMETHYL A column 150x1.0mm

5 µm

300 A

1 mm

150 mm

$597.40
202ME0503

PolyMETHYL A column 200x2.1mm

5 µm

300 A

2.1 mm

200 mm

$556.20
3.54ME0503

PolyMethyl A 5um 300A 4.6 x 35

5 µm

300 A

4.6 mm

35 mm

$370.80
104ME0503

PolyMethyl A 5um 300A 4.6 x 10

5 µm

300 A

4.6 mm

100 mm

$422.30
204ME0503

PolyMETHYL A column 200x4.6mm

5 µm

300 A

4.6 mm

200 mm

$556.20
209ME0503

PolyMethyl A 5um 300A 9.4 x 20

5 µm

300 A

9.4 mm

200 mm

$1,019.70
2521ME0503

PolyMethyl A 5um 300A 21 x 250

5 µm

300 A

21 mm

250 mm

$3,296.00
051ME0510

PolyMethyl A 5um 1000A 1 x 50m

5 µm

1000 A

1 mm

50 mm

$437.75
151ME0510

PolyMethyl A 5um 1000A 1 x 150

5 µm

1000 A

1 mm

150 mm

$592.25
202ME0510

PolyMETHYL A column 200x2.1mm

5 µm

1000 A

2.1 mm

200 mm

$556.20
3.54ME0510

PolyMethyl A 5um 1000A 4.6 x 3

5 µm

1000 A

4.6 mm

35 mm

$370.80
104ME0510

PolyMethyl A 5um 1000A 4.6 x 1

5 µm

1000 A

4.6 mm

100 mm

$442.90
204ME0510

PolyMethyl A 5um 1000A 4.6 x 2

5 µm

1000 A

4.6 mm

200 mm

$525.30
209ME0510

PolyMethyl A 5um 1000A 9.4 x 2

5 µm

1000 A

9.4 mm

200 mm

$1,019.70
2521ME0510

PolyMethyl A 5um 1000A 21 x 25

5 µm

1000 A

21 mm

250 mm

$3,296.00
204ME1203

PolyMethyl A 12um 300A 4.6 x 2

12 µm

1000 A

4.6 mm

200 mm

$324.45
209ME1203

PolyMethyl A 12um 300A 9.4 x 2

12 µm

1000 A

9.4 mm

200 mm

$762.20
204ME1210

PolyMethyl A 12um 1000A 4.6 x

12 µm

1000 A

4.6 mm

200 mm

$324.45
209ME1210

PolyMethyl A 12um 1000A 9.4 x

12 µm

1000 A

9.4 mm

200 mm

$762.20
051ME05006

PolyMethyl A 5um 60A 1 x 50mm

5 µm

60 A

1 mm

50 mm

$437.75
051ME0502

PolyMethyl A 5um 200A 1 x 50mm

5 µm

200 A

1 mm

50 mm

$437.75
051ME0505

PolyMethyl A 5um 500A 1 x 50mm

5 µm

500 A

1 mm

50 mm

$437.75
104ME05006

PolyMethyl A 5um 60A 4.6 x 100

5 µm

60 A

4.6 mm

100 mm

$442.90
104ME0502

PolyMethyl A 5um 200A 4.6 x 10

5 µm

200 A

4.6 mm

100 mm

$442.90