PolyPROPYL A Columns

These materials separate proteins on the basis of hydrophobic character, as does Reversed-Phase Chromatography. HIC uses totally aqueous buffers, maintaining tertiary structure and biological activity. Typically, a sample is eluted with a decreasing gradient of a salt such as sulfate or phosphate. Proteins elute in order of increasing surface hydrophobicity. Surfactants (e.g. CHAPS, octylglucoside) can be added to the mobile phase if necessary. The relative hydrophobic character of PolyPROPYL A is 100.

When To Use HIC:

  1. Characterization of antibodies.
  2. Purification of polypeptides such as glycopeptides and venoms.
  3. Isolation of proteins from crude extracts.
  4. Quality control assay using a method complementary to ion-exchange and Reversed-Phase.
  5. Isolation of integral membrane proteins and their complexes.

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Item #

Item Name

Particle Size

Pore Size

Column Inside Diameter

Column Length

List Price

104PR0315

PolyPROPYL A column 100x4.6mm

3 µm

1500 A

4.6 mm

100 mm

$556.20
051PR0503

PolyPROPYL A column 50x1.0mm (

5 µm

300 A

1 mm

50 mm

$463.50
151PR0503

PolyPROPYL A column 150x1.0mm

5 µm

300 A

1 mm

150 mm

$597.40
202PR0503

PolyPROPYL A column 200x2.1mm

5 µm

300 A

2.1 mm

200 mm

$556.20
3.54PR0503

PolyPropyl A 5um 300A 4.6 x 35

5 µm

300 A

4.6 mm

35 mm

$370.80
104PR0503

PolyPROPYL A column 100x4.6mm

5 µm

300 A

4.6 mm

100 mm

$468.65
204PR0503

PolyPROPYL A column 200x4.6mm

5 µm

300 A

4.6 mm

200 mm

$556.20
209PR0503

PolyPROPYL A column 200x9.4mm

5 µm

300 A

9.4 mm

200 mm

$1,091.80
2521PR0503

PolyPropyl A 5um 300A 21 x 250

5 µm

300 A

21 mm

250 mm

$3,296.00
051PR0510

PolyPropyl A 5um 1000A 1 x 50m

5 µm

1000 A

1 mm

50 mm

$437.75
151PR0510

PolyPropyl A 5um 1000A 1 x 150

5 µm

1000 A

1 mm

150 mm

$592.25
202PR0510

PolyPROPYL A column 200x2.1mm

5 µm

1000 A

2.1 mm

200 mm

$556.20
3.54PR0510

PolyPROPYL A column 35x4.6mm (

5 µm

1000 A

4.6 mm

35 mm

$391.40
104PR0510

PolyPROPYL A column 100x4.6mm

5 µm

1000 A

4.6 mm

100 mm

$468.65
204PR0510

PolyPROPYL A column 200x4.6mm

5 µm

1000 A

4.6 mm

200 mm

$556.20
209PR0510

PolyPROPYL A column 200x9.4mm

5 µm

1000 A

9.4 mm

200 mm

$1,091.80
2521PR0510

PolyPROPYL A column 250x21.0mm

5 µm

1000 A

21 mm

250 mm

$3,553.50
204PR1203

PolyPROPYL A column 200x4.6mm

12 µm

1000 A

4.6 mm

200 mm

$329.60
209PR1203

PolyPROPYL A column 200x9.4mm

12 µm

1000 A

9.4 mm

200 mm

$782.80
204PR1210

PolyPropyl A 12um 1000A 4.6 x

12 µm

1000 A

4.6 mm

200 mm

$324.45
209PR1210

PolyPropyl A 12um 1000A 9.4 x

12 µm

1000 A

9.4 mm

200 mm

$762.20
051PR0315

PolyPROPYL A column 50x1.0mm (

3 µm

1500 A

1 mm

50 mm

$463.50
054PR0315

PolyPROPYL A column 50x4.6mm (

3 µm

1500 A

4.6 mm

50 mm

$437.75
054PR0510

PolyPROPYL A column 50x4.6mm (

5 µm

1000 A

4.6 mm

50 mm

$406.85
109PR0315

PolyPROPYL A column 100x9.4mm

3 µm

1500 A

9.4 mm

100 mm

$1,091.80