PolySULFOETHYL A Columns

This strong cation exchange (SCX) material was developed specifically for HPLC of peptides. At pH 2.7 - 3.0, peptides lose their (-) charges and have net (+) charge. They can be retained by a SCX column such as the PolySULFOETHY A. With a salt gradient, peptides elute in order of increasing number of basic residues. The selectivity complements that of Reversed-Phase (RPC). The capacity is 5 - 10 fold greater than that of RPC and when used in series SCX and RPC will yield sequenceable peptides from most crude mixtures.
The hydrophilic aspartamide surface, when used with high organic mobile phases, allow mixed mode cation-exchange, combining SCX with hydrophilic interaction. Separation is achieved with electrostatic interaction and hydrophilic interaction.

When To Use PolySULFOETHYL A:

Mapping of peptides digests (tryptic, V-8, CNBR, etc) and isoforms.
Purification of Synthetic peptides.
Isolation of natural peptides from crude extracts.
Specific isolation of disulfide-linked peptides from digests.
Specific isolation of C-Terminal peptides.
Assay of N-and C-terminal variant peptides and peptides with blocked termini.
Quality control assay requiring a method orthogonal to RPC.

Most SCX strong cation exchangers use Sulfopropyl (-SP) groups. The hydrophobic interactions are significant with such groups, often resulting in poor recovery and efficiency with hydrophobic peptides. By contrast, PolySULFOETHYL A is based on sulfoethyl groups, and recovery of peptides is generally high and quantitative.

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Item #

Item Name

Particle Size

Pore Size

Column Inside Diameter

Column Length

List Price

202SE0502

PolySULFOETHYL A column 200x2.

5 µm

200 A

2 mm

200 mm

$592.25
050.15SE0303

Polysulfoethyl A 3um 300A

3 µm

300 A

0.15 mm

50 mm

$463.50
102SE0303

POLYSULFOETHYL A COLUMNS

3 µm

300 A

2.1 mm

100 mm

$575.00
104SE0303

PolySULFOETHYL A column 100x4.

3 µm

300 A

4.6 mm

100 mm

$575.00
102SE0315

PolySULFOETHYL A column 100x2.

3 µm

1500 A

2.1 mm

100 mm

$592.25
104SE0315

PolySULFOETHYL A column 100x4.

3 µm

1500 A

4.6 mm

100 mm

$592.25
050.30SE0501

50x0.30mm capillary packed wit

5 µm

100 A

0.3 mm

50 mm

$463.50
150.30SE0501

150x0.30mm capillary packed wi

5 µm

100 A

0.3 mm

150 mm

$566.50
051SE0501

PolySulfoethyl A 5um 100A 1 x

5 µm

100 A

1 mm

50 mm

$458.35
151SE0501

PolySULFOETHYL A column 150x1.

5 µm

100 A

1 mm

150 mm

$597.40
104SE0501

PolySULFOETHYL A column 100x4.

5 µm

100 A

4.6 mm

100 mm

$509.85
204SE0501

PolySULFOETHYL A column 200x4.

5 µm

100 A

4.6 mm

200 mm

$592.25
050.15SE0503

50x0.15mm capillary packed wit

5 µm

300 A

0.15 mm

50 mm

$463.50
150.15SE0503

150x0.15mm capillary packed wi

5 µm

300 A

0.15 mm

150 mm

$566.50
050.30SE0503

50x0.30mm capillary packed wit

5 µm

300 A

0.3 mm

50 mm

$463.50
150.30SE0503

150x0.30mm capillary packed wi

5 µm

300 A

0.3 mm

150 mm

$566.50
051SE0503

PolySULFOETHYL A column 50x1.0

5 µm

300 A

1 mm

50 mm

$463.50
151SE0503

PolySULFOETHYL A column 150x1.

5 µm

300 A

1 mm

150 mm

$597.40
102SE0503

PolySULFOETHYL A column 100x2.

5 µm

300 A

2.1 mm

100 mm

$509.85
202SE0503

PolySULFOETHYL A column 200x2.

5 µm

300 A

2.1 mm

200 mm

$575.00
3.54SE0503

PolySULFOETHYL A column 35x4.6

5 µm

300 A

4.6 mm

35 mm

$401.70
104SE0503

PolySULFOETHYL A column 100x4.

5 µm

300 A

4.6 mm

100 mm

$509.85
204SE0503

PolySULFOETHYL A column 200x4.

5 µm

300 A

4.6 mm

200 mm

$592.25
209SE0503

PolySULFOETHYL A column 200x9.

5 µm

300 A

9.4 mm

200 mm

$1,174.20
2521SE0503

PolySULFOETHYL A column 250x21

5 µm

300 A

21 mm

250 mm

$3,708.00