PolyCAT A Columns

For Flat-Bottom Ports

  • For 1/16" and 1/8" OD Tubing
  • 10-32, 1/4-28 and M6 Threads
  • Improve Transfer Volume Consistency
Upchurch Scientific® VacuTight Fittings are designed to provide airtight, dependable connections under vacuum and low pressure conditions. Many of the VacuTight Nuts have streamlined profiles for use in systems requiring a large number of connections in a small area. Furthermore, the VacuTight Ferrule's small size ensures sufficient nut/thread engagement, even in shallow ports. These features make VacuTight Fittings ideal for combichem, high throughput screening, clinical diagnostic and other automated liquid handling applications.

VacuTight Fittings are available for flat-bottom 10-32, 1/4-28 and M6 threaded ports. The configuration of the VacuTight flat-bottom ferrules prevents over compression and tubing ID reduction that can occur with many coned fittings. The result is more consistent aspirating and dispensing volumes across all system connections.

The 10-32 VacuTight Fittings are for use with 1/16" OD tubing. The 1/4-28 and M6 fittings are available for use with both 1/16" and 1/8" OD tubing. All VacuTight Nuts must be used exclusively with VacuTight Ferrules.

VacuTight Nuts with hex heads can be tightened with a standard 1/4" wrench. All VacuTight Nuts with knurls (finger grip ridges) can be tightened finger tight. Additionally, some of the standard head and headless nuts can be tightened with our Extender Tools. We recommend tool tightening no more than a 1/2 turn past finger tight.

Use any of the 10-32 fittings on this page to make an inline connection with our VacuTight Union. This product is designed for use with 1/16" OD tubing. Or, use your choice of 1/4-28 or M6 VacuTight Fittings with the appropriate union from page 64 to make an inline connection with 1/16" or 1/8" OD tubing.

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204CT0503 , PolyCAT A column 2...

Crude Synthetic Phosphopeptide:
Numerous failure sequences may not be separated by reversed-phase, but the below chromatogram demonstrates the PolyCAT A, when using an acetic acid gradient, will yield a purified target peptide, with remarkable selectivity, superior to Reversed Phase.

The sample was the main fraction collected from reversed-phase step. The product was purified satisfactorily at the cation exchange step. Selectivity is remarkable; deletion of a Thr- residue sufficed to afford nearly baseline resolution from the main product. The elution order was as expected in some cases; Loss of -PO4 or Ser(PO4) would decrease electrostatic repulsion and increase retention time, while loss of two Arg- residues decreased retention. However, the loss of all three Arg- Residues had little effect on retention, while loss of a single Arg- actually increased retention. The indicated deletions were identified by ES-MS. Again , one cannot specify which particular residue is missing if there is more than one possibility.
$540.00

204CT0510 , PolyCAT A column 2...

PEGylated protein (20-30KD) showing alternate PEG sites clearly resolved. The minor peaks near the large peak are proteins where the PEG chain was attached to the lysine residues other than the one involved in the main product.
$545.00

104CT0303 , PolyCAT A column 1...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$556.20

102CT0315 , PolyCAT A column 1...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$556.00

104CT0315 , PolyCAT A column 1...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$556.00

050.15CT0503 , 50x0.15mm capil...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$463.50

050.30CT0503 , 50x0.30mm capil...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$463.50

051CT0503 , PolyCAT A column 5...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$463.50

151CT0503 , PolyCAT A column 1...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$597.40

202CT0503 , PolyCAT A column 2...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$556.20

3.54CT0503 , PolyCAT A 5um 300...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$370.80

104CT0503 , PolyCAT A column 1...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$468.65

209CT0503 , PolyCAT A column 2...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$1,091.80

2521CT0503 , PolyCAT A column ...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$3,553.50

051CT0510 , PolyCAT A column 5...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$463.50

151CT0510 , PolyCAT A column 1...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$597.40

202CT0510 , PolyCAT A column 2...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$556.20

3.54CT0510 , PolyCAT A column ...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$391.40

104CT0510 , PolyCAT A column 1...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$468.65

209CT0510 , PolyCAT A column 2...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$1,091.80

2521CT0510 , PolyCAT A column ...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$3,553.50

204CT1203 , PolyCAT A column 2...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$329.60

209CT1203 , PolyCAT A column 2...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$782.80

204CT1210 , PolyCAT A 12um 100...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$324.45

209CT1210 , PolyCAT A 12um 100...

For Cation-Exchange

The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with little tailing. Binding capacity and recovery are high as well. Operating conditions are similar to those used with other weak cation-exchange (WCX) materials (e.g. CM type). This cation exchange media can be used to separate by charge, or by polarity. Volatile mobile phases with high levels of organic solvents may be used.

Cation-Exchange of Peptides with Volatile Solvents

Ion-exchange is conveniently done with volatile solvents using PolyCAT A™, our weak cation-exchange (WCX) material. A gradient of acetic acid (HOAc) uncharges its carboxyl- groups, and even highly basic peptides are readily eluted. as in the example below. Strong cation-exchange (SCX) materials, such as our PolySULFOETHYL Aspartamide™, are generally too retentive to be used this way.
$762.20